Fig. 2
H3K9me2 and H3K27me3 in chicken embryo gonads. A Immunodetection of H3K9me2 (gray) and germ cell marker (red) in tissue sections. DNA staining (cyan). Scale bar: 10 µm. Quantification of fluorescence intensity in germ and somatic cell nuclei is shown below. Number of analyzed nuclei in 6‑, 8‑, 10‑, and 14‑day-old embryos: for females, 24, 29, 52, and 45 germ cells and 133, 179, 65, and 56 somatic cells, respectively; for males 33, 34, 41, and 38 germ cells and 125, 126, 104, and 112 somatic cells, respectively. B Immunodetection of H3K27me3 (gray) and germ cell marker in tissue sections. DNA staining (cyan). Scale bar: 10 µm. Quantification of fluorescence intensity in germ and somatic cell nuclei is shown below. Number of analyzed nuclei in 6‑, 8‑, 10‑, and 14‑day‑old embryos: for females, 42, 48, 34, and 84 germ cells and 411, 329, 202, and 314 somatic cells, respectively; for males, 31, 21, 34, and 44 germ cells and 434, 224, 225, and 244 somatic cells, respectively