Fig. 1

5mC and 5hmC in chicken embryo gonads. A Immunodetection of 5mC (gray) and germ cell marker (red) in tissue sections. Scale bar: 10 µm. Quantification of fluorescence intensity in germ and somatic cell nuclei is shown below. Number of analyzed nuclei in 6‑, 8‑, 10‑, and 14‑day-old embryos: for females, 44, 50, 60, and 54 germ cells and 74, 101, 68, and 57 somatic cells, respectively; for males, 28, 22, 56, and 34 germ cells and 71, 76, 76, and 47 somatic cells, respectively. B Immunodetection of 5hmC (gray) and germ cell marker (red) in tissue sections. Scale bar: 10 µm. Quantification of fluorescence intensity in germ and somatic cell nuclei is shown below. Number of analyzed nuclei in 6‑, 8‑, 10‑, and 14‑day-old embryos: for females, 48, 27, 49, and 34 germ cells and 76, 81, 54, and 41 somatic cells, respectively; for males, 37, 13, 39, and 33 germ cells and 61, 46, 42, and 48 somatic cells, respectively