Primer | Direction | Sequence (5'→3') | Enzyme site |
---|---|---|---|
Primers for transgenic constructs | Â | ||
vg1.6 kb | Forward | TAAAGCGGCCGC AAGTCTCCGCCCAATAAT | NotI |
vg Δ100 | Forward | TAAAGCGGCCGC AGTTTGTGTGAGAGTGAGC | NotI |
vg Δ300 | Forward | TAAAGCGGCCGC CGTAATTAAAACCGAAGG | NotI |
vg 1.6 kb, vg Δ100, vg Δ300 | All reverse | GCGCTTTCTA GAGAGCATATAGAAGTGGTCGAA | XbaI |
Fab-7 | Forward | TAAAGCGGCCGC GGAATTGTGTGGACGATG | NotI |
Fab-7 | Reverse | GGCGCTTACTAGTGCACAGAGAGTGCAGAAAG' | SpeI |
Primers for mutagenesis | Â | Â | |
vg ΔGAGA | Forward | AAGAGGTTGAACCCCTTGAGGAAACCGGTTTATTTC | - |
vg ΔGAGA | Reverse | GAAATAAACCGGTTTCCTCAAGGGGTTCAACCTCTT | - |
vg Δ1stGT | Forward | CAGGAAACCGGTTTATTTCCACAACTCTGTGCGTG | - |
vg Δ1stGT | Reverse | CACGCACAGAGTTGTGGAAATAAACCGGTTTCCTG | - |
vg Δ1st-2ndGT | Forward | GGTTTATTTCCACAACTCCGTGTGTGCGTTTATGCC | - |
vg Δ1st-2ndGT | Reverse | GGCATAAACGCACACACGGAGTTGTGGAAATAAACC | - |
vg Δ1st-3rdGT | Forward | GT TTATTTCCACAACTCCCGTTTATGCCTGTGTGCG | - |
vg Δ1st-3rdGT | Reverse | CGCACACAGGCATAAACGGGAGTTGTGGAAATAAAC | - |
vg Δ1st-4thGT | Forward | CAACTCCCGTTTATGCCCGCTAGTTTGTGTGAGA | - |
vg Δ1st-4thGT | Reverse | TCTCACACAAACTAGCGGGCATAAACGGGAGTTG | - |