Figure 2From: Proliferating cell nuclear antigen is required for loading of the SMCX/KMD5C histone demethylase onto chromatinEffect of proliferating cell nuclear antigen (PCNA) knockdown on intracellular distribution of endogenous SMCX (Smcy homolog, X-linked (mouse)) protein. 293T cells were transfected with anti-PCNA or control small interfering (si)RNA. Two days after transfection, cells were harvested, then the chromatin fraction was separated from the rest of the lysate and fractions analyzed by western blotting. Ch = chromatin fraction, W-Ch = cell lysate without chromatin.Back to article page